The Bionic Bioreactor Company

BioThrust: The first ever solution to controllable & scalable cell therapy manufacturing.

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Our Bionic Bioreactor

Introducing ComfyCell

A whole new approach to stirred-tank bioreactors for 3D cell therapy manufacturing.

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Maximum Cell Yield
Enabled by minimal shear forces, no foam formation, decreased culture time & high medium efficiency.
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Application Agnostic
Designed to manage applications across various cell types & process modalities, from suspension to adherent cells and from expansion to differentiation.
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Real Scalability
The concept of geometric similarity facilitates linear scale-up from 100 mL to 200 L with consistent process conditions.
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Ready for Commercial
Exclusively medical grade materials or biologically intert components are used for the ComfyCell bioreactor.
Our Artificial Lung

Powered by our unique Membrane Stirrer technology

The BioThrust Membrane Stirrer aerates the cell culture bubble-free & minimizes shear forces with gentle mixing.

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Low Shear Stress
Gas-transfer through diffusion enables shear-stress free cell cultivation while avoiding foam completely.
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Homogeneous Cultivation Conditions
Optimal mixing conditions by CFD-optimized system-design, across all volumes.
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Unsurpassed Gas Transfer
Vast membrane area delivers maximal kLa rates - with constant CO2-stripping.
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Perfusion System
Process intensification via in-situ perfusion system, classic ATF/TFF systems and or Fed-batch process.

Broad Range

Of Applications

Conventional STRs (l) destroy cells through bubble aeration. The ComfyCell (r) gently mixes the media while providing cells with gases and nutrion needed.

Cell Types

83% increase in EVs per mL compared to static control

With THP-1 cells, the 300mL ComfyCell SUB achieved a VCD of 2.19Mn cells/mL (outperforming static culture by 50%) and a EV density of 4.41Bn EVs/mL (outperforming static culture by 83%) - with results confirming stable production of similar EVs.

Cell Types

Viable cell density increased by almost 50%

Human iPSCs were expanded with remarkable success, reaching an impressive expansion factor of 65! Benchmarked against a conventional bioreactor, our system delivered outstanding performance with a threefold increase in total cell numbers, surpassing an incredible 1.6 billion cells!

Cell Types

Successful completion of first human bm-MSC run

Using RoosterBio's human bm-MSC cell line, medium and supplement we were able to confirm expansion with good average viability.

Stay tuned for further updates & publications as we optimize the process!

Cell Types

Impressive >327-fold expansion of iNK cells at 2L

Together with Catalent, iNK cells were expanded in our 300mL SUB and our 2L reusable system, proving the simplicity of upscaling with the ComfyCell. An amazing >327-fold expansion of iNKs (>1000-fold from thaw) while maintaining excellent killing performance confirms the viability of the ComfyCell for commercial iNK production as the only system enabling scales of 10L or more.

Cell Types

Experiments & evaluation ongoing

Stay tuned for further updates & publications

Cell Types

Confirmed 63Mn cells/mL in first high-density perfusion POC

The ComfyCell achieved 63Mn cells/mL in a first proof-of-concept run with HEK293-F cells, maintaining >90% average cell viability without additional optimization strategies. No DO limitations or foaming observed so far.

Stay tuned for further updates & publications as we optimize the process!

Cell Types

Experiments & evaluation ongoing

Stay tuned for further updates & publications

Cell Types

Up to 3mm large organoids cultivated over 35 days in 2L

Compared to conventional runs, our Membrane Stirrer achieved the closest to in-vivo results for iPSC-derived lung organoids our academic partner ever measured, producing up to 3mm large organoids and 100% living cells inside the structure after a process period of 35 days!

Cell Types

More than 35 mn cells/mL without additional oxygen, completely foam free

In a benchmark 10L fed-batch run with a thoroughly optimized conventionally sparged process, the Membrane Stirrer in the first run achieved the same top-notch cell density, but without the need to add any oxygen to the input gas. Furthermore, the usage of anti-foam agents can be completely avoided, since sparging is not required - simplifying handling considerably due to no foam!

Cell Types

More than +200 % increased process yield

Compared to a conventional stirred-tank system, our Membrane Stirrer doubled the achieved cell density and increased production volume by further 30% due to foam avoidance.

Cell Types

Experiments & evaluation ongoing

Stay tuned for further updates & publications

Cell Types

Experiments & evaluation ongoing

Stay tuned for further updates & publications

Process Modalities

Suspension Cells

Leverage the homogeneous, gentle mixing and gas supply of our Membrane Stirrer for a physiological cultivation of your suspension cells (e.g., NK Cells).

Process Modalities

Adherent Cells

Grow your adherent cells (such as MSCs) on microcarriers that are gently kept in motion by the Membrane Stirrer.

Process Modalities

Spheroids (Cell Aggregates)

Manage the size and growth of your three dimensional cell aggregates and spheroids with the precisely controllable shear force induced by the Membrane Stirrer.

Process Modalities

Differentiation Processes

Perform the whole process from cell expansion to differentiation in our ComfyCell Vessel. The currently in development in-situ perfusion system will enable a complete media exchange and the automatic supply of specific substances to trigger differentiation.

Process Modalities

In-situ Perfusion

Leverage the options for continuous supply of nutrients and media exchange through the (currently in development) perfusion system for optimizing cell density and maximizing yield.

Discover Our Application Studies

Our Advocates

“With the BioThrust Membrane Stirrer, we were able to scale up cardiac differentiation of iPS cells in stirred bioreactor with the first attempt. This novel technology paves the way for our translational studies to treat cardiac infarction by stem cell-based cell therapeutics.”
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Prof. Dr. Kurt Pfannkuche
University of cologne
“It is our mission to manufacture extracellular vesicle products from conditioned media of MSCs in a scaled and robust manner. Among others a reliable upstream processing procedure is needed. Here, we focus on the bionic biorector concept of BioThrust, which we consider as the most promising 3D bioreactor process for our purposes."
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Prof. Dr. Bernd Giebel
University Hospital Essen

Experience Comfycell for yourself.

Convince yourself of the new generation of bioreactors - short delivery times, attractive financing models, and process experts by your side make getting started as easy as possible for you.